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Cotton Planting Technology: An Excellent Method For Cotton Breeding And Seedling Raising

2013/3/24 13:51:00 15

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Cotton Planting Planting Technology Cotton Breeding

1, a breeding method of high temperature resistance < a href= > http://cailiao.sjfzxm.com/Matertial/show/default.aspx > cotton hybrid > /a > /p >
 Application (patent) No. 200710086690.7: Abstract: a breeding method for high temperature resistant cotton hybrids belongs to the field of crop resistance breeding. The breeding methods are as follows: 1) select parents and hybridize to get cotton hybrid F1; 2) design the F1 of cotton hybrid according to the randomized block test, investigate the boll number and boll setting rate of the plant in the field; 3) indoor pollen culture in vitro to determine the pollen germination rate and pollen tube growth length of the cotton hybrid F1; 4) use principal component analysis to screen the results, carry out the principal component analysis according to the results obtained by step 2 and 3, and then select the cotton hybrids F1 screened by principal component analysis after the first and second principal component values. A high temperature resistant cotton hybrid with strong boll setting, high yield and strong adaptability is bred by using the method of the invention. The problems such as the falling of the bolls and the yield and quality of lint cotton are seriously affected by the high temperature adversity often occurring in the hybrid cotton growing area of the Yangtze River Valley between July and August. 
 2, ultrasonic assisted Agrobacterium mediated spanformation of cotton germ, 
 Application (patent): 200710063650 
The invention belongs to the field of cotton spangene technology, and discloses a new method for cultivating spangenic cotton by ultrasonic assisted Agrobacterium spanformation of cotton germ. The Agrobacterium tumefaciens of 10ml0.5OD600mm was added to the plastic centrifuge tube or small glass triangular bottle and added the germinated germ to Agrobacterium tumefaciens. The centrifuge tube or small triangular bottle was placed in the center of the ultrasonic cleaner. After treatment with 1-5min ultrasonic wave of 100 to 150W, the treated germ was spanferred to the co culture medium, then washed with sterile water containing cephalosporin, dried up the moisture on the surface of the embryo and spanferred to the recovery medium. The buds were recovered and cultured in light, and then spanferred to the selection medium for subculture. Then the resistant buds were obtained and spanferred to the rooting medium to induce rooting or grafting on the cotton Rootstock to get the regenerated spangenic plants. It includes: inoculating cotton seeds after seed coat sterilization to sterile seedling germination medium, and putting two newly sprouting seeds on Ye Boli's sterile filter paper. The present invention increases the spanient expression rate of GFP (green fluorescent protein) gene in germ cells by 100 times. For the first time, the spangenic plants of island cotton (long staple cotton) are first realized, and a spangenic cotton plant with insect resistant genes is obtained. Molecular and field experiments showed that the foreign gene had been spanferred into the receptor cotton genome and could be stable expressed and inherited. 
 3, Photoperiod Sensitive Genic Male Sterility < a href= "http://www.91se91.com" > cotton gene < /a > resources and its application technology 
 Application (patent): 200610124597.6 
In the special ecological environment: more than 2 varieties of cotton seeds were planted in the polluted farmland during the night, and only a total of only late autumn peaches were found. After being kept for many years, a variety of photoperiod sensitive genic male sterile cotton genes were obtained, and a new breeding method and utilization method for cotton hybrid seed production was opened up. Its characteristics were sunshine duration greater than 13.6nr, male sterility, sunshine duration greater than 13.2nr male fertility, male sterile period, female sterile line, no need for male pollination and direct pollination for hybrid seed production. Abstract: the Photosensitive Genic Male Sterile Cotton gene resource and its application technology are provided in the invention. 
 4, Cotton Cultivar artificial net room resistance to cotton aphid identification method 
 Application (patent): 200610037612.3 
 Abstract: the invention relates to an improved method for identifying cotton varieties resistant to Aphis gossypii by using artificial net room as identification site. In the winter, the soil was deeply turned over, and the eggs were killed by freezing injury. After the spring wheat was planted, the cotton varieties were planted in the artificial net room, and the cotton aphid propagation line was established. When the cotton seedlings reached the 3 leaf stage, the test materials were planted in the artificial net room and the aphids were treated. The aphid damage grades of the tested varieties were investigated in the seedling aphid stage, the aphid stage and the autumn aphid stage respectively. The aphid damage index and the reduction rate of the control were compared with the conventional varieties, and the resistance grade standard of cotton varieties to cotton aphid was established. The specific steps of the method are: establishing artificial net room according to the scale of identification. The invention can significantly improve the accuracy and reliability of cotton varieties for identifying cotton aphids. 
 5, two DREB spancription factors in cotton and their coding genes and application 
 Application (patent): 200610012084.6 
 Abstract: the invention discloses a DREB spancription factor of cotton and its coding gene and application. The aim is to provide a DREB spancription factor derived from cotton and its coding gene and its application in plants with improved resistance to stress. The DRIB class spancription factor is one of the following amino acid residues sequences: 1) SEQID in the sequence table: 1; 2) the sequence of amino acid residues of SEQID: 1 in sequence list is replaced, deleted or added by one to ten amino acid residues, and the protein with resistance to spancription is activated. The invention can be applied to genetic engineering improvement of plant resistance, and has high practical application value. 
 6, cotton cultivation method with sunflower as rootstock > /p >
 Application (patent): 200510094541.6 
The method of harvesting and cultivating cotton is characterized by the germination of sunflower seedlings during the period of Qingming to the beginning of summer. When sunflower seedlings grow 4~6 leaves, cotton seeds are sprouting; sunflower is used as an anvil, and the sunflower incision is deeper to the xylem than the leaf below the surface, and the incision is deep to the xylem. The seeds are placed in the incision of sunflower, and the seeds are bundled. After the cotton seed is germinated, the sunflower is cut on the upper part of the incision to form a cotton seedling with sunflower roots. After five days of seedling raising, the cotton seedlings that are harvested are spanplanted to the field in a routine way, and field management is carried out until harvest is harvested. Abstract: sunflower is used as an anvil. The method can greatly increase cotton yield, enhance cotton resistance to diseases and pests, and resist natural disasters. 
 7, a Cuttage Propagation Method of cotton 
 Application (patent): 200510088964.7 
 Abstract: the invention discloses a cutting propagation method for cotton. The method comprises the following steps: 1) the cotton branches are propagated and rooting in the rooting medium of the cotton regenerated seedlings, or the roots of cotton branches are soaked for 20~28 hours by cutting the roots of cotton branches, and then cuttings are inserted into the cotton Soilless Seedling Substrates for rooting culture; 2) after the cotton branches grow, the roots are spanplanted and the cotton plants are obtained. The two is that the root of fast propagating seedlings is more than that of Miao Zhuang, and the seedlings are few or no; after that, the root system is kept intact; three, it is feasible to implement factory seedling raising, and the seedling substrate has no soil texture and light weight, and it can open the factory to protect seedlings in a concentrated way. The risk is small, the effect is good, and the seedling area is saved. Four, it is possible to carry out seedling spanplanting without carrier. When seedlings are taken on the soilless substrate, the seedlings have no carrier. In addition, if the root promoter is used, the rooting speed of the spanplanted seedlings can be accelerated, the time of returning seedlings can be shortened, and the seedling rate can be increased. Five, the use of branch cuttings instead of cotton seeds can solve the problem of high cost and good degeneration of the hybrid cotton seed production. The invention has the following advantages: first, the cotton branches and pressure can be expanded. 
 8, < a href= "http://www.91se91.com/news/" > cotton hydroponics seedling raising < /a > method 
 Application (patent): 200510031883.3 
 Abstract: the invention relates to a new cotton seedling raising method, which uses floating seedling tray, matrix and nutrient solution to float seedlings in water seedbed, and the emergence rate of superior cotton seeds reaches more than 90%. The cotton seedling root system is developed and has strong viability, and cotton seedlings are neat and uniform. The matching spanplanting technology has the advantages of saving labor, saving cost, simple spanplanting, convenient seedling spanplanting, high spanplanting survival rate, short seedling returning period and flexible spanplanting time.